In a 100x magnification the color of bacteria was PURPLE and the shape is ROUND, GRAPE LIKE STRUCTURE. The other cultured bacteria was gram stain using same procedure at Number 4 The second streak culture was subjected to microscopic test.
The Unknown bacteria is Enterobacter aerogenes To determine the ability of micro organism to detect presence of catalaseĪ selective test for Enteric bacteria that produces evident colonies To determine the ability of micro organism to detect Urease To determine the ability of the organism to split indole from tryptophan To determine the ability of organism to detect enzyme Citrase To determine if bacteria produce gelatinase on fermentation To determine if bacteria can breakdown casein and absorb amino acid on fermentation The Staphylococcus aureus and Enterococcus faecalis are positive to mannitol. The following bacteria are negative to mannitol Bacillus cereus, Bacillus Subtilis, ,Staphylococcus Epidermidis. To determine bacteria that grows in high salt To determination gram reaction of the bacteriumĬrystal violet, distilled water, Iodine, Alcohol Safranin
After 30 sec the mixture was rinse with distilled water, taking extra care not to hit the mixture. The purpose of this is to stain the bacteria. The mixture was soaked with Crystal violet for 30 sec. The mixture of water and Unknown specimen A ) was air dried, then passed lightly 3 times in a heat so that the mixture will stick on the slide. The purpose of the water is to make a pool so that the Unknown specimen can be transferred and spread in the slide. Using a sterilized inoculating loop a drop distilled water in a dry glass slide which was previously soaked in alcohol. These 2 NA plate was incubated to 37 C to grow for next meeting.Īfter 2 days the 2 different NA plates were examined and separately transferred into the microscopic slide to be examine which is the Gram (-) and the gram (+) by using the methods Gram Staining (Tortora, et al. It helps to isolate a possible a gram (+) and a gram (-). The purpose of this is to grow separately the 2 possible bacteria. With a sterilized inoculating loop the colony was separately inoculated to a new set of NA for growth Taking extra care on label and streaking. Since there were 2 distinct color and colony in the NA plate. Isolating a Pure Culture /Colony (Mc Donald, et al p.There was growth on the NA plate and there was 2 distinct color which maybe a manifestation of 2 or more different types of bacteria. The inoculated NA was incubated to 37 C for the next day.Īfter 2 days, The NA plate was analyzed through visual examination as to growth, distinct color and colony. Taking extra care to prevent contamination. The purpose of this procedure is to allow growth of the UNKNOWN 105 in the plate. Using a sterilized inoculating loop a sample was streak on the Nutrient Agar plate. Since the unknown sample contains a Gram (+ ) and a Gram (-), it is just proper to make a growth for the unknown in an agar plate. The Nutrient Agar (NA ) plate was labeled : Student name and Unknown 105. From the knowledge acquired from laboratory exercise by Mc Donald, et al (9), the following procedure and test that has been done are listed in enumerative form as follows This activity includes the application of procedure, technique and skills related to what type of Agar to be use to grow culture, inoculating and sterilizing, reagents to apply, handling of samples away from contamination and the skills in gram staining, handling and use of microscope and the differentiate differential and selective test for gram-negative and gram-positive bacteria.Īn unknown LABELED 105 was given by the Lab Professor. Knowing what organism will give the student an idea of the genus and species that can cause diseases, how it can grow and reproduce including how this type of bacteria can be treated and killed, what type of antibiotic it will be susceptible to. The purpose of this laboratory exercise is to evaluate the student’s knowledge and capabilities in identifying micro organism.
This study is the determination of the UNKNOWN bacterium that was given by Microbiology LAB Professor.
0 kommentar(er)
